Abstract
Selective and rapid detection of biomarkers is of utmost importance in modern day health care for early stage diagnosis to prevent fatal diseases and infections. Among several protein biomarkers, the role of lysozyme has been found to be especially important in human immune system to prevent several bacterial infections and other chronic disease such as bronchopulmonary dysplasia. Thus, real-time monitoring of lysozyme concentration in a human body can pave a facile route for early warning for potential bacterial infections. Here, we present for the first time a label-free lysozyme protein sensor that is rapid and selective based on a graphene field-effect transistor (GFET) functionalized with selectively designed single-stranded probe DNA (pDNA) with high binding affinity toward lysozyme molecules. When the target lysozyme molecules bind to the surface-immobilized pDNAs, the resulting shift of the charge neutrality points of the GFET device, also known as the Dirac voltage, varied systematically with the concentration of target lysozyme molecules. The experimental results show that the GFET-based biosensor is capable of detecting lysozyme molecules in the concentration range from 10 nM to 1 µM.
Highlights
Lysozyme is a ubiquitous enzyme that is widely available in diverse organisms, such as bacteria, bacteriophages, fungi, plants, and mammals
For the selective protein detection, the graphene layer is successively functionalized by pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) and the single-stranded probe DNA (pDNA) designed for lysozyme binding
We have presented aptamer-modified large area chemical vapor deposition (CVD)-grown graphene-field-effect transistor (FET) biosensor for the detection of lysozyme protein biomarker
Summary
Lysozyme is a ubiquitous enzyme that is widely available in diverse organisms, such as bacteria, bacteriophages, fungi, plants, and mammals. Lysozyme is often called the “body’s own antibiotic” (Cheng et al, 2007; Lian et al, 2014). The protein is extensively exploited in food industries for several purposes such as preserving meat and dairy products, as well as fruits and vegetables. In addition to its extensive use in food industry, lysozyme plays a vital role as a biomarker for diagnosing various diseases such as breast cancer (Serra et al, 2002), Alzheimer’s (Sandin et al, 2015), and rheumatoid arthritis (Torsteinsdóttir et al, 1999). GFET for Selective Detection of Lysozyme Biomarker
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