Abstract
This study established a microfluidic chip for the capture of A549 human lung circulating tumor cells via the aptamer-conjugated self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) in the channel. AuNPs are among the most attractive nanomaterials for the signal enhancement of biosensors owing to their unique chemical, physical, and mechanical properties. The microchip was fabricated using soft photolithography and casting and molding techniques. A self-assembly method was designed to attach AuNPs, cell-specific aptamers, and target cells onto the desired area (i.e., SAM area). In this study, the gold microelectrode configuration was characterized by fluorescence microscopy and impedance measurements to confirm the important modification steps. Subsequently, several investigations with the proposed assay were conducted with different cell samples to determine the specific binding ability of the device for A549 adenocarcinoma cancer cells. This work has ensured a simple, convenient, selective, and sensitive approach for the development of biosensors for lung cancer detection during the early stages.
Highlights
Cancer is one of the most typical causes of human death around the world, with about 18 million new cases and up to 10 million cancer deaths each year
A simple microfluidic device was built for the capture of A549 human lung adenocarcinoma cells using a specific aptamer and the self-assembled monolayer (SAM) of AuNPs, because A549 is known as the circulating tumor cells (CTCs) cell line of the early stage in non-small cell lung cancer [22,35]
The AuNPs diameter of 13 ± 1 nm was chosen as the optimal size in our previous works for the protein preconcentrators [34,38,39,40]
Summary
Cancer is one of the most typical causes of human death around the world, with about 18 million new cases and up to 10 million cancer deaths each year. Electrical impedance-based biosensors have been preferably developed for the detection of living cells in microfluidic devices due to their simple fabrication, miniaturization capability, rapid response, and high sensitivity [11,12,13]. A self-assembled monolayer (SAM) of AuNPs on the gold electrodes was designed and developed for cancer cell detection in the microfluidic channel [34] These AuNP features have contributed to the design of simple, rapid, sensitive, and efficient aptasensors. A simple microfluidic device was built for the capture of A549 human lung adenocarcinoma cells using a specific aptamer and the SAM of AuNPs, because A549 is known as the CTC cell line of the early stage in non-small cell lung cancer [22,35]. The obtained results may confirm a simple and rapid assay for the identification of cancer cell lines
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