Abstract
Experimentation demonstrates long-range surface plasmon polariton waveguides as a useful biosensor to selectively detect gram negative or gram positive bacteria in human urine having a low concentration of constituents. The biosensor can detect bacteria at concentrations of 10(5) CFU/ml, the internationally recommended threshold for diagnostic of urinary tract infection. Using a negative control urine solution of bacterial concentration 1000☓ higher than the targeted bacteria, we obtain a ratio of 5.4 for the positive to negative signals.
Highlights
The gold standard for the diagnosis of Urinary Tract Infection (UTI) is the detection of pathogen along with the presence of clinical symptoms
Our experiments demonstrate that long-range surface plasmon polariton (LRSPP) waveguides can selectively detect gram negative or gram positive bacteria in human urine
A first approximation of the sensitivity of the biosensor indicates that it is relevant to the diagnostic of UTI with a detection threshold less than 105 CFU/ml
Summary
The gold standard for the diagnosis of Urinary Tract Infection (UTI) is the detection of pathogen along with the presence of clinical symptoms. We investigate the ability of a label-free long-range surface plasmon polariton (LRSPP) waveguide biosensor to detect and identify bacteria as gram positive or gram negative species in human urine samples. Label-free biosensors such as surface plasmon resonance (SPR) [7], LRSPP resonance [8,9] and LRSPP waveguides [10] can provide detection of bacteria in clean fluids [8,11,12]. They are capable of detection selectivity similar to enzyme-linked immunosorbent assay (ELISA). By measuring and analyzing the optical response of the waveguide with a power detector and an infrared camera, we establish the presence of the target bacteria
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
