Abstract
Dopamine nigrostriatal neurons are important for motor control and may contain a particularly dense population of ryanodine receptors involved in the control of dopamine release. To test this hypothesis, we used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels specifically the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion. On the contrary, no reduction of [3H]-ryanodine binding was observed. The present data indicate that [3H]-ryanodine binding sites present in rat striatum are not preferentially localized in dopaminergic terminals.
Highlights
Dopamine nigrostriatal neurons are important for motor control and may contain a dense population of ryanodine receptors involved in the control of dopamine release
We used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra
Dantrolene, which is mainly used as a skeletal muscle relaxant in the treatment of malignant hyperthermia, acts peripherally by inhibiting the release of calcium from internal stores controlled by ryanodine-sensitive channels [4,5]
Summary
Dopamine nigrostriatal neurons are important for motor control and may contain a dense population of ryanodine receptors involved in the control of dopamine release. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion.
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