Abstract
We optimized a mesencephalic cell culture system to employ low concentrations of 6-hydroxydopamine (6-OHDA) and 1-methyl-4 phenylpyrdium (MPP +), neurotoxins known to trigger oxidative stress in dopaminergic cells. Both 6-OHDA and MPP + at 5 μM reproducibly reduced the survivial of dopaminergic neurons by 50–70% ( p<0.02) without affecting the survival of the non-dopaminergic neuronal population. We found that 1 mM of the non-steroidal anti-inflammatory drug (NSAID), acetylsalicylic acid (ASA), significantly ( p<0.05) increased the survival of dopaminergic neurons exposed to either neurotoxin. The mechanisms underlying neuroprotection by ASA may be of therapeutic import in Parkinson's disease.
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