Abstract
The use of bioactive antioxidants in feed of broiler to mitigate reactive oxygen species (ROS) in biological systems is one of promising nutritional strategies. The aim of present study was to alleviate ROS production in mitochondrial fraction (MF) of meat by supplemented dietary antioxidant in feed of broiler. For this purpose, mitochondria specific antioxidant: α-lipoic acid (25 mg, 75 mg and 150 mg) with or without combination of α-tocopherol acetate (200 mg) used in normal and palm olein oxidized oil (4%) supplemented feed. One hundred and eighty one day old broiler birds were randomly divided into six treatments and provided the mentioned feed from third week. Feed intake, feed conversion ratio (FCR) remained statistically same in all groups while body weight decreased in supplemented groups accordingly at the end of study. The broiler meat MF antioxidant potential was significantly improved by feeding supplemented feed estimated as 1,1-di phenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, 2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS+) and thiobarbituric acid reactive substances (TBARS). The maximum antioxidant activity was depicted in group fed on 150 mg/kg α-lipoic acid (ALA) and 200 mg/kg α-tocopherol acetate (ATA) (T4) in both breast and leg MF. Moreover, TBARS were higher in leg as compared to breast MF. Although, oxidized oil containing feed reduced the growth, lipid stability and antioxidant potential of MF whilst these traits were improved by receiving feed containing ALA and ATA. ALA and ATA showed higher deposition in T4 group while least in group received oxidized oil containing feed (T5). Positive correlation exists between DPPH free radical scavenging activity and the ABTS + reducing activity. In conclusion, ALA and ATA supplementation in feed had positive effect on antioxidant status of MF that consequently diminished the oxidative stress in polyunsaturated fatty acid enriched meat.
Highlights
Polyunsaturated fatty acids e.g. linoleic (C18:2n-6) and linoleinic (C18:3n-3) acids are classified as conditionally essential for human and class mammalian because of deficient enzyme which is responsible to synthesize these acids in plants [1]
It has been observed that ATA deposit linearly in microsomal membrane to stabilize phospholipids, and improved the feed conversion ratio (FCR), growth performance [11] whilst thiobarbituric acid reactive substances (TBARS) was reduced [12]. α-Lipoic acid is a one of the most active biological antioxidant plays a pivotal role in energy metabolism
Maximum weight gain was recorded in group receiving lowest level of ALA (25 mg/kg feed) while minimum weight gain was observed in group that fed on 150 mg/kg ALA with 200 mg/kg ATA feed
Summary
Polyunsaturated fatty acids e.g. linoleic (C18:2n-6) and linoleinic (C18:3n-3) acids are classified as conditionally essential for human and class mammalian because of deficient enzyme which is responsible to synthesize these acids in plants [1]. To reduce or minimized the lipid peroxiation in meat, numerous synthetic and natural antioxidants and antioxidant enriched plant extracts are used in feeds of broiler. One of these antioxidants, α-tocopherol acetate is major lipophilic free radical scavenger in vivo for the protection of membranal lipids [7,10]. Α-Lipoic acid is a one of the most active biological antioxidant plays a pivotal role in energy metabolism It is unique, among antioxidants, owing to its powerful antioxidant properties in both reduced (dihydrolipoic acid) and oxidized (ALA) condition. Antioxidants i.e. ATA and Butylated hydroxyl anisole/Butylated hydroxyl toluene supplementation improved growth and oxidative stability of meat especially in microsome than mitochondria fraction [10]. Resultant meat will be considered healthier and nutritive for human consumption
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