Selective Data-Sampling and Reconstruction of Phase Sensitive 2D NMR Spectra Using Maximum Entropy

Abstract

Whilst one can now determine the three-dimensional structure of small proteins and nucleic acid fragments using two-dimensional (2D) NMR methods, the technique will always suffer from its inherently low sensitivity. This limits the resolution obtainable, in complex 2D NMR spectra of biomolecules. In this paper we review the methods of selective data-sampling and maximum entropy (MEM) data-processing that we have been developing. The results show that they can increase the resolution in 2D NMR spectra. It is hoped that these methods will help extend the use of 2D NMR to cases where otherwise it would be impractical.