Abstract
Catalytic detection of dopamine in a flow-injection system based on a packed bed reactor containing immobilized tyrosinase is demonstrated. Electrochemical or photometric transduction of enzyme reaction products results in limits of detection ( S/N = 3) of 3.5 ± 1.4 μM (16.6 ± 6.6 ng), and 8.4 ± 3.5 μM (40 ± 16.6 ng) respectively. The high catalytic efficiency of the reactor yields a diffusion-controlled system with a sample frequency of 450 h −1 when electrochemical transduction, 25 μl injection volume and 0.9 ml min −1 flow rate are used. The catalytic system can be used in liquid chromatography post-column mode for the selective detection of dopamine in a complex mixture of drugs.
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