Abstract
Short tandem repeat (STR) typing of DNA extracted from various kinds of mixed samples can be difficult because the origin of the blood donor cannot be easily discriminated. We present a method for the extraction of DNA from mixed bloodstains involving plural contributors, after antibody captured cell separation. This method has proven highly successful in the recovery of DNA profiles corresponding to ABO blood type. The methodological steps presented here are simple: leukocyte agglutination by ABO antibody followed by centrifugal separation of agglutinated leukocytes. Using this method, selective DNA can be extracted from mixtures of blood and/or different cells, using cell specific antibodies. The method presented here is applicable to DNA-based identification of various kinds of contaminated samples.
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