Abstract
A common edible mushroom Lentinula edodes, is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from L. edodes mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-α by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides.
Highlights
Research over the past twenty years has shown that mushrooms are a valuable source of numerous bioactive substances [1]
It has been demonstrated that these compounds can affect T and B lymphocytes, natural killer (NK) cells, macrophages, and neutrophils [3,5]
The Lentinula edodes (Berk.) Pegler strain used in our study was ATCC 48085 (ATCC, Manassas, VA, USA)
Summary
Research over the past twenty years has shown that mushrooms are a valuable source of numerous bioactive substances [1]. The best-known components with documented immunomodulatory and antitumor properties are mushroom-derived polysaccharides [2,3,4]. It has been demonstrated that these compounds can affect T and B lymphocytes, natural killer (NK) cells, macrophages, and neutrophils [3,5]. The immunomodulatory properties of fungal polysaccharides depend on their structure, in particular their monosaccharide composition, molecular weight, branching degrees, presence of functional groups, watersolubility, as well as their triple-helical conformation [6]. Lentinan is one of the best known mushroom-derived compounds. It is a highly purified (1-3;1-6)-β-D-glucan, extracted from
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
