Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from Lentinula edodes Mycelium on Human Immune Cells

Beata Kaleta,Radosław Zagożdżon,Jadwiga Turło,Aleksander Roszczyk,Eliza Malinowska,Michał Zych,Marzenna Klimaszewska,Monika Kniotek,Michał Pac

doi:10.3390/biom11121777

Beata Kaleta, Radosław Zagożdżon + Show 7 more

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https://doi.org/10.3390/biom11121777

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Journal: Biomolecules	Publication Date: Nov 26, 2021
Citations: 11	License type: CC BY 4.0

Affiliation: Medical University of Warsaw

Abstract

A common edible mushroom Lentinula edodes, is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from L. edodes mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-α by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides.

Highlights

Research over the past twenty years has shown that mushrooms are a valuable source of numerous bioactive substances [1]
It has been demonstrated that these compounds can affect T and B lymphocytes, natural killer (NK) cells, macrophages, and neutrophils [3,5]
The Lentinula edodes (Berk.) Pegler strain used in our study was ATCC 48085 (ATCC, Manassas, VA, USA)

Summary

Introduction

Research over the past twenty years has shown that mushrooms are a valuable source of numerous bioactive substances [1]. The best-known components with documented immunomodulatory and antitumor properties are mushroom-derived polysaccharides [2,3,4]. It has been demonstrated that these compounds can affect T and B lymphocytes, natural killer (NK) cells, macrophages, and neutrophils [3,5]. The immunomodulatory properties of fungal polysaccharides depend on their structure, in particular their monosaccharide composition, molecular weight, branching degrees, presence of functional groups, watersolubility, as well as their triple-helical conformation [6]. Lentinan is one of the best known mushroom-derived compounds. It is a highly purified (1-3;1-6)-β-D-glucan, extracted from

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