Abstract
Objective: This study was aimed to determine analytical performance and physical character of MI-SPE (Molecular Imprinted Solid Phase Extraction) atenolol using itaconic acid as the functional monomer and to implement the material for the extraction of atenolol in blood serum.Methods: This experiment was performed by determining association constants between monomer-template with UV-Vis spectrophotometer, the synthesis of MI-SPE atenolol using bulk polymerization method, template extraction, evaluation of the adsorption ability and capacity of sorbent, evaluation of sorbent selectivity, and determining their physical character using Fourier Transform Infrared (FTIR) and Scanning Electron Microscope (SEM). In the end, the sorbent then was implemented to extract atenolol in blood serum.Results: Analytical performance showed that MI-SPE sorbent has Imprinting Factor (IF) 10.632 which is the largest number compared to IF when using another beta blocker compound. Physical characterization obtained by MI-SPE using Scanning Electron Microscope (SEM) method showed that MI-SPE morphology has homogeneous pore and number of cavities than its blank. MI-SPE has recovery percentage 92.22 % atenolol when it applied to blood serum spiked with atenolol standard.Conclusion: MI-SPE sorbent made from the itaconic acid monomer in methanol porogen potential to be used for the extraction of atenolol from the blood sample by selectively bind to atenolol.
Highlights
Atenolol is a class of beta-adrenoceptor antagonists or often called beta blockers
MI-solid phase extraction (SPE) atenolol sorbent was made from itaconic acid as a functional monomer with methanol as a porogen by bulk polymerization method so that it can be used as an alternative method of selective sample preparation of atenolol from blood sample after its adsorption characteristics are identified
Instrument that used in this study were centrifugation devices (Yenaco and Hettich), mesh 60 sieves, Fourier Transform Infrared (FTIR) (Shimadzu, IR Prestige-21), ovens (Memmert), UV-Vis spectrophotometers (Analytik Jena, specord 200), digital scales (Ohaus Pioneer), ultrasonic (NEY 1510), water bath (Memmert), and glass tools commonly used in laboratories
Summary
Atenolol is a class of beta-adrenoceptor antagonists or often called beta blockers. Beta blockers are competitive inhibitors and interfere with hormone stimulation action on beta-adrenergic receptors in the nervous system [1]. MIP is cheaper to synthesize and the polymer storage period is very high, and stable [7] The use of this method is increasingly developing in the fields of chemistry and biology, including as artificial antibodies, binding assays, adsorbents for Solid Phase Extraction, and stationary phase chromatography [6]. Previous studies have been conducted on the synthesis of sorbent MIP-SPE atenolol using methacrylate acid (MAA) as a functional monomer [2]. MI-SPE atenolol sorbent was made from itaconic acid as a functional monomer with methanol as a porogen by bulk polymerization method so that it can be used as an alternative method of selective sample preparation of atenolol from blood sample after its adsorption characteristics are identified
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
