Abstract
In this study, ascorbic acid and vanillin were recovered using semi-continuous and continuous operation of an aqueous two-phase system (ATPS) formed by ethanol (C2H5OH) and dipotassium hydrogen phosphate (K2HPO4). For operation, a column reactor was designed, allowing temperature control by a coating system and the circulation of up to two phases simultaneously. The reactor was equipped with a stirring system composed of a shaft and impellers known as vanes. In the experiments, the influence of volumetric flow rate and agitation speed were analysed to enhance recovery of the biomolecules of interest. The best recovery values were obtained in semi-continuous operation at low rotations (60rpm) and high volumetric flow rate (6.0mLmin−1). These parameters were used to verify partitioning using a real food matrix (Dr. Oetker Diet Pudding), achieving recovery of up to 94% for vanillin in the top phase and ascorbic acid in the bottom phase.
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