Abstract

A protein-incorporated silica-based sol-gel composite film was chemically modified onto the surface of an anodized platinum (Pt) wire electrode. The organic/inorganic hybrid sol-gel film was composed of 3-aminopropyl triethoxy silane (APTES), tetraethoxy silane (TEOS) and bovine serum albumin (BSA). The incorporation of BSA was found to be effective to enhance the permselectivity of hydrogen peroxide (H2O2) against possible electrochemical interferents, such as ascorbic acid (AA) and uric acid (UA), probably due to the size-exclusion molecule cut-off mechanism. The anodic current responses of the APTES-TEOS-BSA-based sol-gel film-modified Pt electrode to AA and UA (0.1 mM) were suppressed to less than 1/170 and 1/90 of that to H2O2 (0.1 mM), respectively, suggesting the potential possibility of this film for applications to interference-free oxidase-based amperometric biosensors (e.g., glucose, lactate and glutamate biosensors).

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