Selective affinity of l-histidine immobilized onto poly(ethylene–vinyl alcohol) hollow-fiber membranes for various oligoglucuronans : Influence of the degree of polymerization and the degree of substitution by acetate

Abstract

A new species of oligoglucuronan was previously extracted from a polysaccharide crude fraction produced by a Sinorhizobium meliloti mutant strain, by chromatography on l-histidine immobilized onto poly(ethylene–vinyl alcohol) (PEVA) hollow-fiber membranes. Until now, only one specific species of oligoglucuronan was selectively adsorbed on the membranes with immobilized histidine. In this work, the specific membranes, commonly used to purify proteins, were tested on different non-ionic and anionic carbohydrate components. We determined only anionic oligosaccharides are selectively retained on these membranes. The affinity between the l-His–PEVA membranes and oligoglucuronans varying in the degree of polymerization and the degree of substitution by acetyl residues was studied.