Abstract
Periodontal tissue damage, accompanied by the degradation and destruction of periodontal tissue collagen, is one of the most clinically common complications and difficulty self-repair in patients with diabetes. Human periodontal ligament stem cells (PDLSC) are the undifferentiated mesenchymal cells that persist in the periodontal ligament after development of periodontal tissue and the ability of PDLSC osteogenic differentiation is responsible for repairing periodontal tissue defects. However, the reasons of high glucose environment in diabetic patients inhibiting PDLSC to repair periodontal tissues are unclear. To address these issues, we propose exposing PDLSC to high-sugar mimics the diabetic environment and investigating the activity of osteogenic differentiation and adipogenic differentiation of PDLSC. At the cellular level, high glucose can promote the adipogenic differentiation and inhibit osteogenic differentiation to decrease the self-repair ability of PDLSC in periodontal tissues. Mechanistically at the molecular level, these effects are elicited via regulating the mRNA and protein expression of C/EBPβ, PPAR-γ.
Highlights
Diabetes is a metabolic disorder characterized by hyperglycemia, its complications involving many organs such as cardiovascular, eye, kidney and foot, to name a few[1, 2]
We further understand the biological activity of periodontal ligament stem cells (PDLSC) under high glucose environment and the reason of PDLSC have a limit for periodontal tissue repair due to its selective adipogenic differentiation
The results showed that: (1) high glucose can inhibit the osteogenic differentiation of PDLSC; (2) high glucose can promote the formation of lipid droplets and increase the adipogenic differentiation of PDLSC; (3) High glucose can promote the lipidation process by increasing the expression of CCAAT-enhancer-binding protein β (C/EBP β) and peroxisome proliferator- activated receptor γ (PPAR-γ) mRNA
Summary
Diabetes is a metabolic disorder characterized by hyperglycemia, its complications involving many organs such as cardiovascular, eye, kidney and foot, to name a few[1, 2]. Studies have shown that the major diabetic microangiopathies such as diabetic retinopathy eventually lead to the loss or even loss of vision[3]. Diabetes can lead to skin wound healing delay or gangrene, leading to diabetic foot disease[4]. Periodontal tissue damage is one of the most clinically common complications in patients with diabetes[5]. The relationship of pathogenesis in diabetes and periodontal tissue damage is similar, both are multifactorial diseases[6]. Due to diabetes can cause glucose metabolism disorder, microangiopathy, end products of glucose-induced endings, and tissue healing ability, resulting in periodontal microcirculation, eventually lead to periodontal tissue damage[8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
