Abstract
The changes taking place in an actively denitrifying population when glucose was replaced by malate as sole carbon source were investigated. Each condition induced a different metabolic pattern. With glucose as carbon source, glycolytic enzymes and those associated with the pentose phosphate shunt were highly active, while the citric acid cycle enzymes were more active when malate was substituted as carbon source. Cytochromes were more abundant during the malate phase. Denitrification was far more efficient while malate was being supplied than was the case with glucose.
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