Selection of tomato tissue cultures able to grow on ribose as the sole carbon source

Abstract

When tomato (Lycopersicon esculentum Mill.) callus or cell cultures were placed on media containing ribose as the sole carbon source, the tissues turned dark brown and ceased growth. However, after approximately 60 days bright green tissue able to grow on ribose emerged from 3 % of the brown necrotic callus tissue pieces plated. The selected tissue was highly organized, consisting of leafy primordia and associated meristematic tissues, sustained growth on ribose, and demonstrated the capacity to regenerate whole plants for at least 3 years. Cultures able to grow on ribose could not be selected from liquid suspension cultured tomato cells or from callus which had been mechanically macerated into cell aggregates containing less than approximately 100 cells. Plants regenerated from ribose adapted cultures were abnormal, having shortened internodes and thicker greener leaves. Regenerated plants were both male and female sterile.