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Abstract
Bacterial persister cells are a subpopulation of isogenic bacteria with characteristics of reduced metabolic activity and multidrug antibiotic resistance. Our lab had previously proved that Vibrio splendidus could form persister cells both naturally and after stimulation. However, the conditions for the waking up of V. splendidus persister cells remain marginal. In this study, the carbon sources that could wake up V. splendidus persister cells were selected from 20 amino acids and eight saccharides. The result showed that L-glutamic acid, L-aspartic acid, L-arginine, L-phenylalanine, L-leucine, maltose, D-galactose, sorbitol, mannose, N-acetyl-D-glucosamine, D-glucose, and D-fructose could wake up the V. splendidus persister cells. The chemotaxis activity of both exponential cells and regrown persister cells on plate containing each of the selected carbon source are also high. The existence of the selected carbon source can affect the antibiotic susceptibility of V. splendidus. When L-glutamic acid, L-aspartic acid, L-phenylalanine, and D-glucose were separately added into the cultured V. splendidus simultaneously with tetracycline, V. splendidus could be completely eliminated, while the addition of L-alanine and D-galactose could not. Our study suggested that V. splendidus persister cells could revive in the presence of specific carbon sources, and the addition of these exogenous nutrients could increase the tetracycline susceptibility of V. splendidus.
Highlights
Persister cells are a little potion of bacterial cells which are temporarily resistant to multidrug on account of a metabolic transient alteration [1]
Our result showed that V. splendidus persister cells revived on M9 minimal plate supplemented with D-galactose and maltose at 24 h and they revived on mannose and sorbitol at 36 h, but V. splendidus persister cells slowly revived on M9 agar plate with separate D-fructose, N-acetyl-D-glucosamine, and D-glucose when the resuscitation time increased to 48 h
V. splendidus persister cells can revive in the context of the amino acids including L-glutamic acid, L-aspartic acid, L-phenylalanine, L-leucine and L-arginine, and saccharides including D-galactose, maltose, mannose, sorbitol, D-fructose, N-acetyl-D-glucosamine, and D-glucose
Summary
Persister cells are a little potion of bacterial cells which are temporarily resistant to multidrug on account of a metabolic transient alteration [1]. To explore whether saccharides could be used as carbon source to resuscitate V. splendidus persister cells, eight saccharides, including maltose, D-glucose, D-ribose, mannose, D-galactose, sorbitol, N-acetyl-D-glucosamine and D-fructose, were separately added into M9 minimal medium.
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