SELECTION OF STAPHYLOCOCCAL IMMUNOGENES TO OBTAIN HYPERIMMUNE POLYCLONAL SERUM

Abstract

Complicated staphylococcal infections: sepsis, meningitis, endocarditis and other requires the development of new generation biological agent with high medicinal property, devoid of adverse events. In this direction, development of microbial cell antigen, anti-hemolytic, anti-leukocidin and anti-hyaluronidase immunoglobulin is promising, which needs staphylococcal immunogene for immunization of producers. Selection of staphylococcal strains was based on the study of morphological, cultural and enzymatic activity, determination of sensitivity to novobiocin and staphylococcal bacteriophage. Among 102 laboratory and clinical staphylococcal strains, following were selected: a) high plasmocoagulating, with hemolytic property, mannitol decomposition and proteolytic property, also St. aureus 24 sensitive to novobiocin and staphylophage and St. epidermidis 6 strains devoid of the mentioned property; b) Thermally inactivated staphylococcal strains, due to their stable characteristics; c) Staphylococcal: alfa-anatoxin, PV-leukocidin and hyaluronidase. Mentioned immunogens were used for priming and immunization of producer-animals (goats). First cycle of immunization has been administered. In the immune serum, the activity of antibodies against the used immunogens is determined by immunoenzymatic analysis, based on „Sandwich-ELISA“ and in passive hemagglutination reaction, using diagnostic test-systems.