Abstract
Seedlings from Canadian alfalfa cultivars Algonquin and Apica were selected on the basis of ability to regenerate plants from hypocotyl callus. A total of eleven genotypes were selected, resulting in regeneration frequencies of 4.5 and 11.7% for Algonquin and Apica, respectively. Regeneration capacity within these cultivars was also compared with cultivars and breeding lines for which regeneration frequencies have previously been documented. Two genotypes (A1 93 and Ap 20) exhibited prolific embryogenesis from hypocotyl and petiole callus and also regenerated plants from suspension culture. Flowering and seed set have been observed for regenerated plants.
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