Abstract
MicroRNAs (miRNAs) are promising biomarkers in cancer research. Quantitative PCR (qPCR), also known as real-time PCR, is the most frequently used technique for measuring miRNA expression levels. The use of this technique, however, requires that expression data be normalized against reference genes. The problem is that a universal internal control for quantitative analysis of miRNA expression by qPCR has yet to be known. The aim of this work was to find the miRNAs with stable expression in the thyroid gland, brain and bone marrow according to NanoString nCounter miRNA quantification data. As a results, the most stably expressed miRNAs were as follows: miR-361-3p, -151a-3p and -29b-3p in the thyroid gland; miR-15a-5p, -194-5p and -532-5p in the brain; miR-140-5p, -148b-3p and -362-5p in bone marrow; and miR-423-5p, -28-5p and -532-5p, no matter what tissue type. These miRNAs represent promising reference genes for miRNA quantification by qPCR.
Highlights
The discovery of the small non-coding RNA lin-4 in Caenorhabditis elegans in 1993 established a base for a new line of research in molecular biology [1]. 2,654 human miRNA genes are currently deposited in the miRBase database [2]
The aim of this work was to find the miRNAs that could be used as potential reference genes from among those miRNAs that are stably represented in the thyroid gland, brain and bone marrow, according to nCounter miRNA Assay data
All biological material was obtained in compliance with the legislation of the Russian Federation, and written informed consent was provided by all the patients, all the data were depersonalized
Summary
The discovery of the small non-coding RNA lin-4 in Caenorhabditis elegans in 1993 established a base for a new line of research in molecular biology [1]. 2,654 human miRNA genes are currently deposited in the miRBase database [2]. The discovery of the small non-coding RNA lin-4 in Caenorhabditis elegans in 1993 established a base for a new line of research in molecular biology [1]. 2,654 human miRNA genes are currently deposited in the miRBase database [2]. Some of them are false-positive entries [3]. This led to the need of alternative databases that could give a true view of all miRNAs in existence. One of such databases is MirGeneDB 2.0: in 2020, it contained 556 validated human miRNAs [4]. MicroRNAs play an important regulatory role in many organisms in many biological processes. There is little doubt that aberrant miRNA expression may entail disease initiation and progression [5]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
