SELECTION OF NUTRIENT SOLUTION FOR CLONAL MICRO-PROPAGATION OF INTRODUCED ROSE VARIETIES (ROSA L.) IN THE CONDITIONS OF THE FOREST-STEPPE OF UKRAINE

Abstract

The article deals with the study on the inclusion of biotechnology in the selection of roses. Roses are heterozygous plants and by seed propagation give splitting. Therefore, to accelerate the reproduction of new and existing starting material, clonal micropropagation was included in the study. The in vitro reproduction process is influenced by various factors. It should be noted that, in the initial stages it is influenced by the age of the mother plant and the season of material isolation and even from which part of the plant the explant was selected and its size. The next step is to pay attention to the sterilizing agent and the sterilization exposure period. One of the main factors influencing the clonal micro-propagation is the plant genotype, the hormonal factor and the composition of the nutrient medium. At the last stage of clonal propagation, the main role is played by the condition of the regenerating plant and physical factors. The valuable varieties on decorativeness and permanent flowering capacity as well as various originators were used in the research. The article gives a brief description of valuable genotypes of roses. Highlighted data on the optimal composition of the nutrient in which the largest number of formed micro shoots was achieved. It was investigated that when the biotechnological unit was included in the selection of roses, it was determined that the optimal concentration of cytokinins, namely BAP is 0.5 mg/l and 1.0 mg/l. At such concentrations, no callus formation was observed in most cases, and the indicators of new micro shoots of genotypes and their length were the most optimal. In addition, it should be noted that the main factor in clonal micro-propagation is the genotype of the original sample. Thus, in our studies, the best genotypes for clonal micro-propagation with the largest number of micro shoots produced are Tchaikovski and Koralovyi Surpryz varieties.