Abstract
Reversed-phase column liquid chromatography with on-line electron-capture detection can conveniently be carried out if miniaturized equipment (1 mm I.D. columns) is used. As eluents, mixtures of methanol or dioxane and water are preferred to acetonitrile—water mixtures. With the former two modifiers, working at flow-rates of ca. 50 μl min −1 does not create any problems with mixtures containing between 0 and 50% water. At lower flow-rates, even pure water can be used as eluent. The addition to the mobile phase of small amounts of polar compounds such as phosphoric acid, can be tolerated. Examples of successful applications include the analysis of barbiturates, benzodiazepines, nitroaromatics, C 1C 2 halogenated aliphatic hydrocarbons, and chlorophenols.
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