SELECTION OF ISSR MOLECULAR PRIMERS FOR STUDIES OF GENETIC DIVERSITY IN Handroanthus impetiginosus (MART. EX DC.) MATTOS

Jéssica Maia Alves Pimenta,Cristiane Gouvêa Fajardo,Mauro Vasconcelos Pacheco,Francival Cardoso Felix,Jéssica Sabrina Ovídio De Araújo

doi:10.1590/1983-21252022v35n124rc

Jéssica Maia Alves Pimenta, Cristiane Gouvêa Fajardo + Show 3 more

Open Access

https://doi.org/10.1590/1983-21252022v35n124rc

Copy DOI

Abstract

ABSTRACT Handroanthus impetiginosus is a tree species with ecological and economic potential. Despite that, in the Brazilian market, its wood is heavily exploited in the illegal trade. Therefore, studies on genetic diversity are necessary in order to propose strategies for conservation of this species. Thus, the aim of this study was to select Inter Simple Sequence Repeat (ISSR) primers for genetic diversity studies applied to the forest population of H. impetiginosus. For this, 30 ISSR molecular primers were tested in 30 individuals, evaluating the total number of loci, polymorphism rate and polymorphic information content, as well as marker index and resolving power. Eight primers were selected for having a better amplification pattern, which provided 62 loci. The polymorphic information content of the primers ranged from 0.34 to 0.49, while the marker index (MI) averaged 3.20, with resolving power (RP) of 2.40, Nei’s diversity (He) of 0.35 and Shannon index (I) of 0.52. The results show that the primers UBC 807, 809, 818, 824, 857, 860, 873 and 881 are efficient for quantifying the genetic diversity of H. impetiginosus. These results can contribute to supporting strategies aimed at the conservation of this species and selection of parent trees.

Highlights

Handroanthus impetiginosus (Mart. ex DC.) Mattos (Bignoniaceae) is a native tree species, whose wood is widely used due to its high density, durability, resistance against insects and fungi, which make it heavily exploited in Brazil (SILVA JUNIOR et al, 2017)
Due to the need for more information for the conservation of H. impetiginosus to assist in future population conservation strategies and enable the analysis of the genetic structure of the population, the objective of this study was to select Inter Simple Sequence Repeat (ISSR) primers for genetic diversity studies applied to forest populations of H. impetiginosus
Among the 30 primers tested for H. impetiginosus, eight ISSR primers (UBC 807, 809, 818, 824, 857, 860, 873 and 881) showed satisfactory amplification patterns and loci resolution quality according to previously established criteria

Summary

Introduction

Illegal exploitation and the intense pressure of logging on this species stimulate efforts towards the conservation of existing populations (SILVA JUNIOR et al, 2017). These factors contribute strongly to the registration of H. impetiginosus in the Red Book of the Brazilian Flora (Livro Vermelho da Flora do Brasil) as a species of high economic value and decline of natural populations (verified or projected) (MARTINELLI; MORAES, 2013). Genetic variability directly affects the evolutionary dynamics of populations. The analysis of genetic diversity in forest populations provides information for the conservation and maintenance of species, contributing to the development of strategies for the conservation of genetic resources in situ or ex situ (FAJARDO; VIEIRA; MOLINA, 2014). Molecular primers are useful for the evaluation of genetic variability between populations and between individuals of the same population (VIEIRA et al, 2015)

Objectives

Methods

Results