Abstract
Astragalus exscapus ( Fabaceae ) is a melliferous, perennial plant, which fits within a rarity type characterized by having small populations of relatively high habitat specificity (Rabinowitz, 1981). Astragalus exscapus L. ssp. transsilvanicus (Schur) Nyar . is an endemic subspecies, which has been identified in the Transylvanian basin in 24 populations. In order to establish conservation strategies and to characterize polymorphism of the threatened subspecies, genetic and morphologic analysis has to be implemented. Our study describes the application and optimalization of SRAP procedures to A . exscapus, including sampling, DNA extraction, PCR amplification and electrophoresis. Sequence Related Amplified Polymorphism (SRAP) molecular marker system is going to be used on eight different populations of A. exscapus ssp. transsilvanicus to identify the degree of polymorphism which is essential for the evolution of species. The methodology was tested and optimized successfully in two repetitive experiments. The results confirmed the suitability of the DNA extraction method to be applied and the optimization of the SRAP technique for the large-scale studies we are performing on this subspecies.
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