Selection of bacteria and plant seeds for potential use in the remediation of diesel contaminated soils

Abstract

Enumeration and recovery of the dominant bacteria from a chronically fuel contaminated soil has been investigated. Bacterial counts from these polluted soils ranged between 0.70x10(8) and 28.20x10(8) CFU/g soil. Three different types of bacterial colonies have been recovered on the agar plates. Biochemical examination of the recovered bacteria revealed that they mainly belonged to the genus Pseudomonas, Micrococcus and Bacillus. Turbidity, cell biomass (dry weight basis), and physical appearance determined the growth of these bacteria on diesel. A noticeable decline in alfalfa (Medicago sativa) seeds germination of 15-30% was shown at 500 mg/kg diesel or higher. Under these contaminated conditions, fescue grass (Cyndon dactylon) exhibited a higher viability than alfalfa indicating that C. dactylon seeds are relatively tolerant to diesel and can possibly be used in phytoremediation of diesel contaminated soils. Results of diesel phyotoxicity to seed germination of these two plants were based on filter paper media and therefore; should be considered as first indication only. Extrapolation of such results to actual soil conditions should be cautiously approached taking into account diesel sorption on soil and mechanisms of its bioavailability.