Abstract
The aim of this study is to select some Bacillus isolates which are capable of yielding several beneficial enzymes and antagonism to Vibrio parahaemolyticus causing the EMS shrimp disease. In this study, we isolated and screened total of 54 Bacillus isolates from 30 mud, water and shrimp samples at shrimp ponds in Soc Trang province. Among these, 19 isolates were resistant against Vibrio parahaemolyticus strains causing the EMS shrimp disease via two testing methods. Three of them including NA2B13, NA10B2, NA8B1 isolates showed strongresistance and strong one to three kinds of extracellular enzymes to produce. Result of 16S rDNA sequencing and MALDI -TOF showed that NA2B13 and NA8B1 were Bacillus subtilis and NA10B2 was B. amyloliquefaciens. These two species were regarded safe and having potential applications in the production of biological products to prevent EMS shrimp disease.
Highlights
Khả năng kháng một số chủng vi khuẩn Bacillus với V. parahaemolyticus gây bệnh early mortality syndrome (EMS) bằng phương pháp khuếch tán trên lỗ thạch
The aim of this study is to select some Bacillus Three of them including NA2B13, NA10B2, isolates which are capable of yielding several NA8B1 isolates showed strongresistance and beneficial enzymes and antagonism to Vibrio strong one to three kinds of extracellular enzymes parahaemolyticus causing the EMS shrimp to produce
We isolated and screened MALDI -TOF showed that NA2B13 and NA8B1 total of 54 Bacillus isolates from 30 mud, water were Bacillus subtilis and NA10B2 was B. and shrimp samples at shrimp ponds in Soc Trang amyloliquefaciens
Summary
Chủng vi khuẩn V. parahaemolyticus gây hội chứng EMS nghiên cứu hiện đang được lưu trữ tại Chi nhánh Viện Ứng dụng Công nghệ tại TP.HCM. Chủng Bacillus spp.phân lập từ mẫu đất, mẫu nước và hệ tiêu hóa tôm khỏe được lấy trong khu vực nuôi tôm khỏe tại tỉnh Sóc Trăng
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