Abstract
The bactericidal antibiotic nalidixic acid can be used to select in cultures of Klebsiella pneumoniae and other penicillin-resistant enteric bacteria any auxotrophic and carbohydrate-negative mutant. Nalidixic acid treatment is superior to penicillin or d-cycloserine treatment, since no cross-feeding by lysed cells is possible, and it is simple to handle.
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