Selection and characterization yeast potential from pineapple for bioethanol production using some sugar sources

Abstract

Bioethanol can be produced through fermentation process of sugars based on carbohydrate biomass such as lignosellulosa by microorganism such as yeast. Yeast changes sugar as a carbon source to produce ethanol and CO2 in anaerobic condition. Pineapple as a fruit commodity that is widely cultivated in Indonesia contain high sugar and carbohydrates so it might contain yeast which has the potential in bioethanol production. This research aims to select and characterize potential yeast from pineapple for bioethanol production from some sugars. The method included fermentation test, ethanol production (measurement of reduction sugar, dry cell biomass, and ethanol contain), and molecular identification. Yeast utilize substrates as carbon source for producing cell or ethanol. Ethanol production influenced by substrates, microorganism, and total cell. The results showed that NHC3 used as selected isolate based on the degree of coloration in Oxidative Fermentative Media. The largest ethanol production was 10,63 g/L obtained from mannose where the highest value of substrate utilization efficiency was 95%. The value of yield product is 0,27 g bioethanol/g substrate and yield biomass is 0,14 g biomass/g substrate. BLAST result shows NHC3 had homology >99% with Saccharomycetales and Kodamaea ohmeri.