Abstract
ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.
Highlights
Bovine genital campylobacteriosis (BGC) is a subclinical disease with very subtle signs in large herds, especially in the absence of zootechnical controls
C. fetus subsp. venerealis is transmitted to bovines sexually, but it can be transmitted through contaminated semen or fomites, especially during semen collections
Sequencing of the extracted DNA after amplification of blue colonies isolated from the titration of the non-amplified eluate of the third cycle yielded 88 sequences that after translation showed the selection of 12 different heptapeptides from the phage library. These phages were nominated according to the sequence of the seven amino acids and had their frequencies calculated, according to table 1
Summary
Bovine genital campylobacteriosis (BGC) is a subclinical disease with very subtle signs in large herds, especially in the absence of zootechnical controls. It is a venereal disease that infects the female genital tract. Bulls are asymptomatic animals when they carry the etiological agent of the disease, the bacterium Campylobacter fetus subsp. The foreskin is the habitat for the microorganism, which remains predominantly within the crypts of the epithelium and in the glands of the penis and distal urethra. Venerealis is transmitted to bovines sexually, but it can be transmitted through contaminated semen or fomites, especially during semen collections. Venerealis infects the vagina, cervix, uterus, and oviducts, colonizing and causing inflammation in the genital tract. Venerealis causes inhibition of embryo implantation in the uterus or Approved 12.30.20 Returned CR-2020-0945.R1 by the author 02.24.2C1 iência C. fetus subsp. venerealis causes inhibition of embryo implantation in the uterus or Approved 12.30.20 Returned CR-2020-0945.R1 by the author 02.24.2C1 iência
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