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Abstract
Summary A lysine plus threonine resistant line was isolated by screening embryoid populations induced from a suspension culture of Daucus carota treated with N-methyl-N’-nitro-N-nitrosoguanidine.Amino add analysis showed that embryoids and callus of the resistant line contained at least 8 times more free threonine and 2.5 times more free isoleucine than the wild type.Lysine levels remained unchanged, methionine was hardly detectable both in wild type and resistant line tissues.The overproduction of threonine and isoleucine has been correlated with a decreased sensitivity to feedback inhibition of the lysine sensitive aspartate kinase.Resistant plants are cultivated further for genetical analysis.
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