Abstract
The purpose of the current study is to investigate the use of ultrafiltration membrane for the fractionation of phenolic compounds from subcritical water grape pomace extract and the separation of these compounds from other co-extracted components. The extract was assayed in a cross-flow apparatus against eleven membranes with molecular weight ranging from 100 to 2 kDa. Monitoring of the process was executed by determining performance parameters and retention coefficients of proteins, polysaccharides, sugars, phenolic and anthocyanin classes. Results indicated that retention of solutes was affected, not by size exclusion, but primarily by severe fouling phenomena due to polar solutes adsorption on the membrane surface. With the exception of the separation obtained between polymeric and monomeric proanthocyanidins, polysulfone membranes were not able to fractionate phenolic classes. Membranes starting of 20 kDa and over retained high percentages (>60%) of polysaccharides and proteins.
Highlights
A significant quantity of grape derivatives are generated worldwide by the wine industry. These byproducts are an important source of antioxidant molecules such as polyphenols, ranging from 2.5 to 7.8 g 100 g-1 dry weight (DW) (Spigno and De Faveri, 2007)
The results obtained showed the permeability that is the slope of Jw in function of transmembrane pressures (TMP)
The current study suggests that the purification of phenolic compounds recovered from grape extract is possible using UF
Summary
A significant quantity of grape derivatives are generated worldwide by the wine industry These byproducts are an important source of antioxidant molecules such as polyphenols, ranging from 2.5 to 7.8 g 100 g-1 dry weight (DW) (Spigno and De Faveri, 2007). Grape byproducts contain quantities of different phenolic compounds, important for their role in plant physiology and are regarded as noteable components of human nutrition. The latter idea is backed by numerous studies indicating interesting antioxidant capacity; cardioprotective, neuroprotective and anticancer (Craft et al, 2012; Kähkönen and Heinonen, 2003; Quideau et al, 2011; Stintzing et al, 2002). The authors established that polysulfone membranes were able to separate phenolic compounds from pectin fractions. Zagklis and Paraskeva (2015) proposed a purification method for the separation of grape marc phenolic compounds coupling UF (100 kDa) and NF 480 Da) with resin adsorption/desorption
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