Selected Haematological and Biochemical Parameters of Blood in Rats After Subchronic Administration of Vanadium and/or Magnesium in Drinking Water

Abstract

The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg V/mL; group III, magnesium sulphate (MS) at a concentration of 0.06 mg Mg/mL; and group IV, SMV-MS solution at the same concentrations. The control group received at this time deionized water to drink. It was calculated that group II ingested with drinking water about 10.7 mg V/kg b. w./24 h, group III 6 mg Mg/kg b. w./24 h, and group IV about 9 mg V and 4.5 mg Mg/kg b. w./24 h. The exposure to vanadium alone (group II) led to a statistically significant decrease in body weight gain, food and fluid intakes. Moreover, in the same group of rats a statistically significant decrease in the RBC count, Hb concentration, MCV, and MCH values was demonstrated. Additionally, a statistically significant decrease in the plasma L-ascorbic acid concentration and a significant increase in MDA concentration in blood in this group were found. Instead, after the administration of magnesium alone (group III), a statistically significant decrease in the fluid intake and in the L-ascorbic acid concentration in plasma was noted. Furthermore, in the same group of rats a statistically significant increase in Hb level and in the plasma magnesium concentration was demonstrated. Two-way analysis of variance (ANOVA) did not reveal the interactions between V and Mg.