Abstract
Biological activity of iron introduced into nutrient medium as a suspension of iron oxide nanoparticles (MNPs) synthesized by the laser target evaporation was investigated. Exophiala nigrum (E. nigrum) eukaryotes were grown either in the presence or in absence of MNPs. De-aggregated suspensions of Fe2.75O4 MNPs were added in concentrations of 1 to 104 maximum permissive dose (MPD, being 0.3 mg/L of Fe ions in water). Cells were exposed for 24 to 96 hours periods and then plated onto a solid medium. The effect of MNPs was evaluated by the change in the number of cells during exposure and the number and morphology of the colonies. For 1-10 MPD yeast showed unaltered characteristics. For 100 or 1000 MPD for 72 hours of exposure and above the number of cells increases up to 30 times in comparison with the control. A pronounced stimulating effect was revealed at 104 MPD of iron. A significant excess of the number of cells was observed for the first day. At exposures of 72 and 96 hours the differences in the number of cells in comparison with the control were 11 and 30 times, respectively. A change in the colonies morphology was observed at 100 MPD concentration.
Highlights
Recent focus on wide employment of magnetic nanoparticles (MNPs) in wide spectrum of biomedical applications [1,2] requires the study of the features of their interactions with biocomponents, in particular with eukaryotic organisms
The results obtained in the present work require further development for better understanding of the mechanism of stimulation of cell divisions of E. nigrum yeasts and revealing the nature of the heterogeneity of the population according to the morphological criteria induced by the presence of MNPs
We suggest that E. nigrum cages contain iron oxide in the form of MNPs [2, 10,16], which makes yeast an attractive model object at the stage of development of suspensions for hyperthermia, thermal ablation and magnetic biosensing
Summary
Recent focus on wide employment of magnetic nanoparticles (MNPs) in wide spectrum of biomedical applications [1,2] requires the study of the features of their interactions with biocomponents, in particular with eukaryotic organisms Iron oxides such as magnetite (Fe3O4) and maghemite (γ-Fe2O3) are well known as materials with a high degree of biocompatibility, a sufficiently high saturation magnetization at room temperature and a high Curie temperature [3]. For pharmaceutical products containing nanocomponents, there is a requirement for additional testing of samples of each lot just prior to application of the drug [7] These circumstances are the reason that the toxic, cytotoxic and genetic effects of MNPs require further study and refinement. This study was focused on the morpho-functional reactions of E. nigrum eukaryotic microorganisms in response to the effect ofFe2.75O4 iron oxide magnetic nanoparticles in form of electrostatically stabilized water-based suspension
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