Selected elements of extracellular matrix of the skin in diabetes and insulin resistance

Abstract

PurposeReconstruction of the skin extracellular matrix is a physiological phenomenon occurring on a continuous basis. The aim of this study is to evaluate the content of basic enzymes preventing oxidative stress: superoxide dismutase 2 and 3 as well as catalase, the content of hyaluronic acid, and the activity of N-acetyl-β-d-hexosaminidase and β-d-glucuronidase in the skin of rats used as animal models of diabetes and insulin resistance, before and after the treatment. Materials and methodsThe study was conducted on a group of sexually mature male Wistar rats divided into 7 groups of 10 animals. Insulin resistance was induced by feeding the rats with a high-fat diet, and diabetes was induced by a single injection of streptozotocin. Chosen groups of rats were treated with insulin or metformin. After 8 weeks, we excised a fragment of shaved dorsal skin from anesthetized rats in each group. ResultsIn the course of diabetes and insulin resistance, an intensified defensive activity of cells against the oxidative stress was observed in the undamaged skin, expressed by an increase in the relative content of superoxide dismutase 2 and 3, catalase and the activity of N-acetyl-β-d-hexosaminidase and β-d-glucuronidase. Diabetes and insulin resistance cause similar skin damage, as there are no differences in the relative contents or specific activities of the examined parameters. ConclusionsInsulin and metformin improve the quality of the skin in rats with diabetes and insulin resistance, by restoring the content of hyaluronic acid to the healthy skin level.