Segregation patterns of several morphological characters and RAPD markers in interspecific hybrids between Dianthus giganteus and D. carthusianorum

Abstract

The segregation ratio of morphological characters (15 quantitative and 4 qualitative) and random amplified polymorphic DNA (RAPD) markers in 55 interspecific hybrids between Dianthus giganteus and D. carthusianorum and their parents were studied. Interspecific hybrids exhibited either intermediate characteristics between parents shorter, fewer characteristics than parents except for stem length and height of corolla where heterosis was found. Segregation of four qualitative characters such as profile of the lower part of the corolla, stigma color, the main secondary color of petal blade, and arrangement of individual flowers, were exhibited as either characteristics of female parent or male parent. Two hundred and sixteen polymorphic RAPD bands detected in 55 interspecific hybrids were divided into four types: AB type (both-parental type) which was presented in both female parent and male parent, Ab type (female parental type) which was presented in only female parent, aB type (male parental type) which was presented in only male parent, and ab type (non-parental type) which was presented in only hybrids except their parents. The number of AB, Ab, aB, and ab type bands were 33 (15.3%), 56 (26.0%), 50 (23.1%), and 77 (35.6%), respectively. The proportion of parental type bands to non-parental type bands was two to one and only 54.6% of four type bands coincided with expected ratio.