Segment-specific expression of tight junction proteins, claudin-2 and -10, in the rat epididymal epithelium

Abstract

Tight junctions are known to be related to the variance by segment of the luminal fluid microenvironment of the epididymis. We examined the expression of claudins that regulate the paracellular permeability of ions, solutes, and water through tight junctions in the rat epididymis. RT-PCR analysis showed that claudin-2 and -10 were expressed in a segment-specific manner among fifteen claudins examined. Immunofluorescence microscopy demonstrated that both claudins showed developmental stage- and segment-specific subcellular localizations in the epididymal epithelium. Claudin-2 was detected principally in the apical junctional region in the initial segment at postnatal day 7 and week 10 but lost its expression in all the distal segments including the caput, corpus, and cauda. Claudin-10 was detected principally in the apical junctional region in all segments on postnatal day 7 but only in the initial segment--especially along the entire lateral membrane--at 10 weeks. In freeze fracture electron microscopy, well-developed intramembranous particle strands were observed on the P face and complementary grooves with a few particles on the E face at 10 weeks. Many particles were occasionally observed on the grooves in the E face in the initial segment and distal caput. Tight junction strands were continuous and impermeable to lanthanum nitrate on postnatal day 7, suggesting the establishment of functional tight junctions. These segment-specific expressions of claudin-2 and -10 may contribute to creating the specific luminal fluid microenvironment which is necessary for the transport, maturation, and storage of spermatozoa.