Abstract

AbstractBecause of the increasing global population, changing climate, and consumer demands for safe, environmentally friendly, and high‐quality food, plant breeders strive for higher yield cultivars by monitoring specific plant phenotypes. Developing new crop cultivars and monitoring through current methods is time‐consuming, sometimes subjective, and based on subsampling of microplots. High‐throughput phenotyping using unmanned aerial vehicle‐acquired aerial orthomosaic images of breeding trials improves and simplifies this labor‐intensive process. To perform per‐microplot phenotype analysis from such imagery, it is necessary to identify and localize individual microplots in the orthomosaics. This paper reviews the key concepts of recent studies and possible future developments regarding vegetation segmentation and microplot segmentation. The studies are presented in two main categories: (a) general vegetation segmentation using vegetation‐index‐based thresholding, learning‐based, and deep‐learning‐based methods; and (b) microplot segmentation based on machine learning and image processing methods. In this study, we performed a literature review to extract the algorithms that have been developed in vegetation and microplots segmentation studies. Based on our search criteria, we retrieved 92 relevant studies from five electronic databases. We investigated these selected studies carefully, summarized the methods, and provided some suggestions for future research.

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