Abstract
Aims of this research were to get the right dosage of Plant Growth Regulkator (PGR) and Coconut Water (CW) for shoot multiplication of Kulo chrysanthemums. The study was conducted using a factorial design of three factors namely IAA (0.0; 0.5 and 1.0 ppm), BAP (0, 0.5 and 1.0 ppm) and coconut water (0.0 and 30%). The explants used were clean micro cuttings and were cultured on Murashige and Skoog (MS) media. The media was added IAA, BAP and coconut water. The result showed that the best shoot multiplication of Kulo chrysanthemums was the MS media using combination of 0.5 ppm and 1.0 of IAA, 0.5 ppm of BAP and 30% of coconut water. Good rooting was revealed by the combination of IAA 0.5 and 1 ppm with 30% coconut water and BAP 1 ppm and 30% coconut water.
Highlights
Flower of Kulo Chrysanthemums (Lokon white) is white chrysanthemum that have been designated as chrysanthemum varieties from Tomohonby decree ofI Indonesian Minister of Agriculture in 2012 number 2276 / kpts / SL-120 / VIII / 2012 (Antaranews.com Manado, Manado Express, 2015)
Used Material were clean Kulo chrysanthemum which was cultured to media MS0 (MS without Plant Growth Regulator (PGR)), Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), coconut water and Murashige and Skoog (MS) basic media
The results of this study showed that the height of chrysanthemum kulo increased by coconut water treatment
Summary
Flower of Kulo Chrysanthemums (Lokon white) is white chrysanthemum that have been designated as chrysanthemum varieties from Tomohonby decree ofI Indonesian Minister of Agriculture in 2012 number 2276 / kpts / SL-120 / VIII / 2012 (Antaranews.com Manado, Manado Express, 2015). Propagation through tissue culture is determined by many factors such as genetic factors and media including Plant Growth Regulator (PGR). Kulo chrysanthemum propagation through tissue culture needs to be explored using Plant Growth Regulator (PGR) which can increase shoot multiplication, the growth of shoots and plantlet until acclimatization process. It is necessary to study the use of PGR in propagation through tissue culture techniques to obtain large number of shoots and better growth. Mandang et al (1997) showed that the use of coconut water on media MS was increasing orchid protocorm. Research Mandang et al (1997) resulted that partly substituted MS media with coconut water was able to increase shoot dry weight chrysanthemum and chlorohill content. The use of coconut water is important for tissue culture
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