Abstract
Recirculating aquaculture systems (RAS) for anadromous fish such as salmonids often require salinity changes during the production cycle. However, high or variable salinity can disrupt the biological nitrification process, which can be detrimental to the fish due to the accumulation of toxic ammonia or nitrite. Thus, it is vital to maintain sufficient nitrification capacity in RAS during salinity changes. This study investigated whether seeding with salinity-acclimated carriers in the freshwater start-up phase could increase the salinity tolerance of nitrifying bioreactors. Moving bed biofilm reactors (MBBR) were started with virgin carriers and seeded with mature biofilm carriers acclimated to freshwater (F), brackish water (B, 12‰ salinity), or a 1:1 mix of both (FB). All duplicate reactors were started up in freshwater and the salinity was increased to seawater (~32‰ salinity) after ~7 weeks. While F and FB had a 65–75% decrease in ammonia oxidation capacity immediately after seawater transfer, B had only a ~20% reduction. After 40 days in seawater, ammonia oxidation recovered completely and became similar in all treatments. However, nitrite accumulation was observed in all the treatments several days after the salinity increase, with the least accumulation in B and the highest in F. The type of seeding influenced the composition of the nitrifying microbial community in the new biofilms (in the freshwater phase). However, the composition in the treatments became similar after ~6 weeks in seawater. The findings indicate that seeding with brackish water biofilm carriers is a potential strategy for accelerating start-up and improving the acclimation of freshwater nitrifying bioreactors to salinity stress. However, nitrite oxidizing bacteria may require a longer period for salinity adaptation. Thus, it is important to closely monitor the nitrite concentration for a prolonged period (several days or weeks) after a salinity increase.
Highlights
Recirculating aquaculture systems (RAS) are a technology for pro ducing fish in land-based systems with water treatment and reuse
Nitrification is a two-step process performed by two distinct microbial guilds: 1) ammonia oxidizing microorganisms (AOM, includes ammonia oxidizing bacteria (AOB) and archaea (AOA)) that perform the first step of oxidizing ammonia to nitrite; and 2) nitrite oxidizing bacteria (NOB) that convert nitrite to nitrate (Madigan et al, 2018)
After seawater transfer, ammonia oxidation rate (AOR) and nitrite oxidation rate (NOR) in both F and FB reduced by 60–65%, whereas it reduced only by ~20% in B
Summary
Recirculating aquaculture systems (RAS) are a technology for pro ducing fish in land-based systems with water treatment and reuse. Salinity changes can disrupt the performance of the water treatment processes in the RAS, especially the nitrification process (Chen et al, 2006). Nitrification is a biological process where the toxic ammonia produced by the fish is successively converted to nitrite and nitrate. Freshwater bioreactors can undergo a severe reduction in nitrification rate when the salinity is increased (Bassin et al, 2011; Gonzalez-Silva et al, 2016; Kinyage et al, 2019; Moussa et al, 2006; Navada et al, 2019). As a reduction in the nitrification efficiency can quickly lead to ammonia/nitrite accumulation and a consequent risk to the fish, it is necessary to develop strategies to increase the salinity tolerance of RAS bioreactors
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