Abstract
Carrot (Daucus carota L.) is among the most economically important vegetable crops worldwide. Seedborne bacterial pathogens of carrot cause important damages to seed quality and yield of plants. In this study, seedborne bacteria were determined on some carrot seeds sown in Turkey. Seeds of different orange and black color varieties of carrot were collected from Eregli and Kasınhanı districts of Konya province, where the highest carrot production is reported. Subsamples of 10,000 seeds were soaked in 100 ml sterile saline (0.85% NaCl) with 0.02% Tween 20 overnight at 5 °C, YDCA, KB, MKM, MD5A and mTBM media were used for bacterial isolation and bacterial morphological characterization. Biochemical, physiological and molecular methods were used for the identification of the bacterial isolates. Pathogenicity tests of strains were performed on orange color carrots, and pathogenic strains induced a hypersensitive reaction in tobacco plants. The 60 pathogenic and saprophytic bacterial strains were obtained belong to Pseudomonaceae, Bacillaceae, and Xanthomonadaceae families. There were twenty-three seed samples on 5 different orange carrot cultivars Maestro, Bolero, Sireco, Natuna and Romans, and 11 black carrot genotype of traditional cultivar ‘Eregli’. Two pathogenic bacteria were defined as Xanthomonas hortorum pv. carotae and Pseudomonas viridiflava at different percent infestation ratios (17.39-18.18%) and (9.09-13.04%) on orange and black carrot seed samples. To the best of our knowledge, it is the first report of P. viridiflava on carrot seeds in Turkey.
Highlights
Carrot (Daucus carota L.) is consumed as edible roots; usually, orange in color but red, white, purple, and yellow varieties exist
Total 60 bacterial isolates were obtained from twenty three seed lots on 5 different orange carrot cultivars, Maestro, Bolero, Sireco, Natuna and Romans, and 11 black carrot genotype of traditional cultivar ‘Ereğli’ in Konya province
Mainly two pathogenic bacteria were identified as X. h. pv. carotae (16 strains) and P. viridiflava (13 strains) on orange and black carrot seeds at different percent infestation ratios (Table 3)
Summary
Carrot (Daucus carota L.) is consumed as edible roots; usually, orange in color but red, white, purple, and yellow varieties exist. The world’s carrot seed industry is concerned directly with a seed-borne disease i.e., bacterial blight, caused by X. hortorum pv. Despite being able to detect the seed-borne infection, development of seed contamination thresholds for specific regions of carrot production (Umesh et al, 1998), and the availability of seed treatments to eliminate seed-borne inoculum (Howard et al, 1994; Pscheidt and Ocamb, 2001), the carrot industry is still facing losses due to this disease (Kuan et al, 1985; Umesh et al, 1996). Carotae in carrot seeds in Turkey are very scarce and the pathogen was reported only once by Demir and Ustun (2001) in one carrot seed sample in Aegean Region. This study aimed to determine the seed-borne bacterial pathogens on seeds of black and orange carrots in the areas with highest production, i.e., Kasınhanı town and Eregli district of Konya
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
