Seed traits correlate with herbicide resistance in Italian ryegrass (Lolium perenne ssp. multiflorum).

Abstract

Italian ryegrass (Lolium perenne ssp. multiflorum) is one of the major winter annual weeds worldwide. In this research, diversity for seed morpho-physiological traits such as seed weight, seed size, awnedness, dormancy, speed of germination, and seed vigor among Italian ryegrass populations collected from the Texas Blacklands region were assessed, and potential association with herbicide resistance was investigated. A high degree of diversity was observed among the populations for 100-seed weight (125-256 mg), seed length (4.8-6.6 mm), awn length (0-6 mm), and total seedling length (9-14 cm at 21 days after seed germination). Inter-population range for seed dormancy was higher in the freshly harvested seed (31-85%), which reduced to 18 to 62% at 9months after harvest. Populations with high initial seed dormancy (> 70% dormancy) released dormancy at a faster rate than the low dormancy group (< 40%). Percent survival status to multiple postemergence herbicides was positively correlated with 100-seed weight and fresh or initial seed dormancy. Early emerging cohorts are easily controlled by pre-plant tillage and preemergence herbicides, whereas late emerging cohorts (facilitated by seed dormancy) are exposed to postemergence herbicides wherein greater opportunities exist for resistance evolution, likely explaining the occurrence of high seed dormancy in Italian ryegrass populations resistant to postemergence herbicides. High seed weights can further allow seedling emergence from greater burial depth, thereby exposing more seedlings to postemergence herbicides and increasing the likelihood of resistance evolution. Results provide unique insights into the association between seed traits and herbicide resistance in this species. © 2021 Society of Chemical Industry.