Abstract
The purpose of a seed train is the generation of an adequate number of cells for the inoculation of a production bioreactor. This is time- and cost-intensive: From volumes used for cell thawing or cell line maintenance the cell number has to be increased. The cells are usually run through many cultivation systems which become larger with each passage (e.g. T-flasks, roller bottles or shake flasks, small scale bioreactor systems and subsequently larger bioreactors. Single-use systems may be applied and systems which are inoculated at a partly filled state and culture volume is increased afterwards by medium addition). The production bioreactor is inoculated out of the largest seed train scale.
Highlights
The purpose of a seed train is the generation of an adequate number of cells for the inoculation of a production bioreactor
The tool is suitable for different cell lines via entering corresponding model parameters, medium and seed train information
Seed train optimization is possible regarding cell passaging at optimal Space-Time-Yield (STY) or other optimization criteria [1]
Summary
The purpose of a seed train is the generation of an adequate number of cells for the inoculation of a production bioreactor. This is time- and cost-intensive: From volumes used for cell thawing or cell line maintenance the cell number has to be increased. The production bioreactor is inoculated out of the largest seed train scale. Motivation A seed train offers space for optimization, e.g. choice of optimal points in time for cell passaging from one scale into the larger one. When designing a new seed train, the volumes of the cultivation scales may be open for optimal choice
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