Abstract
Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.
Highlights
Proteolytic enzymes are involved in many aspects of plant physiology and development and are the subject of intensive research interest
In this paper we present the isolation and characterization of partial FeAP12 cDNA from buckwheat encoding for typical plant aspartic proteinases (AP)
In order to isolate cDNA encoding for buckwheat aspartic proteinase, PCR reaction was performed using degenerated primers and total cDNA from developing buckwheat seeds having in mind that aspartic proteinase activity was found to be the most prominent in that organ (Timotijević et al 2003)
Summary
Proteolytic enzymes are involved in many aspects of plant physiology and development and are the subject of intensive research interest. Fifty one genes were identified in the Arabidopsis genome as coding putative aspartic proteinases Those sequences were grouped into three categories: typical aspartic proteins, nucellin-like and atypical plant aspartic proteinases (Faro and Gal, 2005). For the great majority of plant APs biological functions are still hypothetical and represent a challenging field of research These enzymes are believed to participate in various proteolytic processes during seed development and germination, including the maturation of storage proteins and the initiation of their hydrolysis (D’Hondt et al, 1993; Guilloteau et al, 2005). We analyzed its expression in different buckwheat organs as well as throughout different stages of seed maturation
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