Seed Quality Evaluation by Tetrazolium Staining of &amp;lt;i&amp;gt;Parkia multijuga&amp;lt;/i&amp;gt; Benth

Marcelle Auday Costa,Noemi Vianna Martins Leão,Hugo Alves Pinheiro,Elizabeth Santos Cordeiro Shimizu

doi:10.4236/as.2018.95040

Abstract

The purpose of this study was to develop a staining pattern through tetrazolium test to evaluate seed viability of Parkia multijuga Benth (fava-arara-tucupi). The seeds were scarified, soaked into water and distilled for 48 hours for tegument removal and then immersed in a 0.05%; 0.1%; and 0.3% tetrazolium solution for 6; 18 and 24 hours at 35°C, in the dark. The evaluation was performed on the basis of stain intensity and aspect of the tissues. Based on color patterns, the seed images were classified in eight viability classes. Those results were compared with the germination test in a laboratory. The treatment in concentration of 0.1% for 6 hours was considered adequate for the specie, considering the adequate staining and time for analysis.

Highlights

Parkia multijuga known fava-arara-tucupi or pinho-cuiabano is a woody specie from the Central Amazon
The purpose of this study was to develop a staining pattern through tetrazolium test to evaluate seed viability of Parkia multijuga Benth
The results expressed the viability of the seeds in concentrations 0.05 % for 6 and 24 hours and 0.1% and 0.3% for 6, 18 and 24 hours, because they did not differ significantly from the germination test (Table 1)

Summary

Introduction

Parkia multijuga known fava-arara-tucupi or pinho-cuiabano is a woody specie from the Central Amazon. It is found in both solid ground and high floodplain argillic soil [1]. It presents a moderate annual production of viable seeds [2] with physical dormancy imposed by a hard and waterproof tegument [1] [3]. The use of high quality seeds which is essential for the success of the introduction of any culture and forest operations depends on seedling quality and the consequent success of a reforestation program. There are internal quality control programs developed by seed companies that aim for fast and efficient tests to determine the viability of seed lots

Objectives

Methods

Results

Conclusion