Seed priming improves the emergence potential, growth and antioxidant system of Moringa oleifera under saline conditions

Abstract

Moringa oleifera is a multipurpose plant which is now being promoted as a fodder crop. The present study was conducted to induce the tolerance in moringa plants to emerge and grow under saline conditions. For this, moringa seeds were primed with aerated water (hydropriming) and moringa leaf extract (MLE) for 12 and 24 h and studied for its emergence, potential growth behaviour, mineral composition, chlorophyll contents and antioxidant activities in comparison with unprimed seeds to investigate the physiological changes in moringa plants under saline conditions. The seeds were sown in plastic pots filled with acid washed sand at four salinity levels (3, 6, 10, 14 dS m−1) in a completely randomized design with three replications. It was found that salinity >6 dS m−1 reduced the emergence, growth and vigour of moringa plants but hydropriming (12 h) enhanced moringa emergence at 10 dS m−1 followed by MLE priming (12 h). Maximum aboveground biomass and photosynthetic pigments were recorded when the seeds were hydroprimed (12 h) but maximum root length and number of roots were found in MLE primed (12 h) moringa plants. Significant decrease in K+:Na+ ratio with increasing salinity levels resulted in low K+ and Mg2+ uptake and Na+ toxicity in moringa leaves which resulted in reduced chlorophyll contents at 14 dS m−1 but a significant increase in chlorophyll a and b contents and total phenolics were found in hydroprimed seeds (12 h) while the antioxidant activities of superoxide dismutase, peroxidase and catalas were improved by MLE priming (12 h). This study concludes that moringa emergence and growth performance can be improved by hydropriming under saline conditions.