Seed Germination and Seedling Growth Responses to Different Sources and Application Rates of Hydrothermal Carbonization Processed Liquid

Yuhang He,Kris Pruski,Bishnu Acharya,Lord Abbey,Quan He

doi:10.5539/sar.v10n4p1

Abstract

Hydrothermal carbonization processed liquid (HTCPL) is a by-product of hydrothermal carbonization of biomass, which is used sparingly as natural fertilizer. A study was performed in the Faculty of Agriculture, Dalhousie University (Canada) between June 2019 and April 2020 to evaluate the elemental composition of HTCPL derived from three different biomass feedstock; namely, seafood compost; buckwheat (Fagopyrum esculentum), and willow (Salix babylonica). Different HTCPL application rates (0-10%) were tested on seed germination and seedling growth of pea (Pisum sativum), sunflower (Helianthus annuus), pac choi (Brassica rapa subsp. chinensis), kale (Brassica oleracea var. sabellica) and lettuce (Lactuca sativa). Elemental composition was higher in the HTCPLs compared to their respective feedstocks except for nitrogen. The 5% and 10% willow HTCPL with a pH between 3.8-4.0 inhibited seed germination and seedling growth compared to the other treatments with a pH range between 4.6-5.8. Kale, lettuce and sunflower radicle and hypocotyl growth were promoted following treatments of their respective seeds with seafood compost HTCPL while pea radicle and hypocotyl lengths were best promoted by 5% buckwheat and 10% seafood compost HTCPLs. Comparatively, 0.5% willow HTCPL increased surface area of seedling radicles while 1% willow and 0.5% buckwheat HTCPLs increased surface area of hypocotyls, irrespective of plant species. The distinction among the treatments was demonstrated on a 2-dimensional principal component analysis biplot that explained 89% of the variations in dataset. Overall, buckwheat HTCPL proved to be more effective at increasing seed germination and seedling growth compared to the other HTCPLs. The inhibitory effect of willow HTCPL at high application rate (5-10%) were obvious for all plant species. A comprehensive non-targeted chemical profile of HTCPL will help to explain mechanisms.

Highlights

Hydrothermal carbonization (HTC) is a thermochemical process used to convert organic materials at temperatures between 180o and 350°C to value-added products (Saetea and Tippayawong, 2013)
In addition to N, P, K and Mg were remarkably high in buckwheat HCTPL followed by willow HCTPL and seafood Hydrothermal carbonization processed liquid (HTCPL) (Table 1)
The results suggested that the different HTCPLs did not significantly (P>0.05) influence seed germination potential compared to the control but was significantly (P=0.008) reduced by willow HTCPL

Summary

Introduction

Hydrothermal carbonization (HTC) is a thermochemical process used to convert organic materials at temperatures between 180o and 350°C to value-added products (Saetea and Tippayawong, 2013). Biomass such as plants, composts, and sewage sludge are commonly used as feedstocks for HTC (Saetea and Tippayawong, 2013; Funke, 2015; Yao et al, 2016; Idowu et al, 2017). HTCPL extracted from rosemary (Rosmarinus officinalis) showed significant antioxidant activity due to the presence of IS-α-pinene and eucalyptol in the starting feedstock (Ma et al, 2013)

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