Seed cryopreservation of Passiflora species

Deizimary Stella De Araújo,Severino De Paiva Sobrinho,Petterson Baptista Da Luz,Leonarda Grillo Neves

doi:10.1590/2317-1545v38n3154922

Deizimary Stella De Araújo, Severino De Paiva Sobrinho + Show 2 more

Open Access

https://doi.org/10.1590/2317-1545v38n3154922

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Abstract

Abstract The aim of this study was to evaluate the cryopreservation of seeds of Passiflora species, using different cryoprotectants. The completely randomized design was used with four treatments (T1 - DMSO at 7%; T2 - 0.3 M sucrose; T3 - storage in NL2 without cryoprotectants; T4 - control) and five replications of 50 seeds. The seeds were stored for 120 hours in NL2 (-196 °C), and thawing was conducted in a water bath (37 °C) for 20 minutes, proceeding with the germination and vigor tests. The data were submitted to ANOVA and Tukey's test (5% of probability) using the SISVAR program. In P. mucronata, P. suberosa and P. edulis seeds, the responses obtained were favorable to cryopreservation without the use of cryoprotectants in regards to P. micropetala, there was a considerable loss of germination potential after storage in NL2. Given these circunstances, cryopreservation can be used for long-term conservation of Passiflora mucronata, P. suberous and P. edulis, without the use of cryoprotectants.

Highlights

Several wild species of Passiflora have shown a major potential for use in genetic improvement programs, with nutritional, ornamental, and medicinal characteristics, as a source of resistance to diseases, as rootstock, in terms of the quality of the fruits and its adaptability for culture (Faleiro et al, 2011)
According to Delanoy et al (2006), several authors have shown that the germination of passion fruit trees may go from ten days to three months, showing a low germination percentage and irregularity in the formation of seedlings
The water content of the seeds used for the cryopreservation varied from 6.2% for P. micropetala to 12% for the P. mucronata and P. edulis species

Summary

Introduction

Several wild species of Passiflora have shown a major potential for use in genetic improvement programs, with nutritional, ornamental, and medicinal characteristics, as a source of resistance to diseases, as rootstock, in terms of the quality of the fruits and its adaptability for culture (Faleiro et al, 2011). In Brazil, the genetic erosion in Passiflora species has occurred in a significant manner, due to factors such as the growing use of new areas of the Central-Northern regions for agricultural and industrial purposes (Faleiro et al, 2011). In that sense, Fowler and Bianchetti (2000) observed that some species show dormant seeds, that may be related to physical factors (tegument impermeability to water and gas). On studies for different species, it was observed that tissues that surround the embryo, such as the endosperm and perisperm, may restrict the radicular growth (Welbaum and Bradfort, 1990)

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