Abstract
1. Three ATP extraction methods for Echinochloa crus-galli var. praticola imbibed seeds were tested: 1) boiling intact seeds 2) boiling cracked seeds and 3) grinding seeds with or without 5% TCA. With the two boiling methods a very small amount of ATP was detected. The grinding method accomplished in a very short time using Triton X-100 as an extract solution for ATP gave the best results.2. Three methods of cleaning for various durations were compared in E. crus-galli var. praticola seeds: 1) dipping in 70% ethyl alcohol (EtOH), 2) dipping in 1% NaClO solution and 3) exposure to formaldehyde gas. The results showed that dipping in 70% EtOH or exposing to formaldehyde gas for 3 minutes is an appropriate means of cleaning E. crus-galli var. praticola seed for ATP assay.
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