Abstract
This is a brief preliminary report of results indicating that we have been successful in sedimenting poliomyelitis virus, one of the smallest of the ultramicroscopic viruses,1 from clear aqueous suspensions by means of ultracentrifugation in vacuum. The machine employed is similar in design to that recently described by Bauer and Pickels,2Thus far we have obtained complete results on 2 experiments. In both, the material subjected to ultracentrifugation consisted of a 25% suspension of glycerinated pooled virus cords in physiological saline. The lipoids in these suspensions were removed by ether extraction and the aqueous fraction was centrifuged in an Angle centrifuge at 3000 r.p.m. for at least an hour. In the first experiment the resultant fluid was water-clear to the eye; in the second, very slightly opalescent. Eight cc. of the clear suspension were placed in each of a series of celluloid tubes seated in the rotor.In the first experiment, the rotor was in motion for a total of 4 hours. Stroboscopic de...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
