Abstract
This is a brief preliminary report of results indicating that we have been successful in sedimenting poliomyelitis virus, one of the smallest of the ultramicroscopic viruses,1 from clear aqueous suspensions by means of ultracentrifugation in vacuum. The machine employed is similar in design to that recently described by Bauer and Pickels,2Thus far we have obtained complete results on 2 experiments. In both, the material subjected to ultracentrifugation consisted of a 25% suspension of glycerinated pooled virus cords in physiological saline. The lipoids in these suspensions were removed by ether extraction and the aqueous fraction was centrifuged in an Angle centrifuge at 3000 r.p.m. for at least an hour. In the first experiment the resultant fluid was water-clear to the eye; in the second, very slightly opalescent. Eight cc. of the clear suspension were placed in each of a series of celluloid tubes seated in the rotor.In the first experiment, the rotor was in motion for a total of 4 hours. Stroboscopic de...
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